NAD+ (Nicotinamide Adenine Dinucleotide)

Buy NAD+ Research Grade | Pure Cellular Energy Coenzyme for Longevity Research | PrimaLab Peptide

NAD+ (Nicotinamide Adenine Dinucleotide) is a critical cellular coenzyme found in every living cell and one of the most widely studied molecules in cell biology — occupying a central position in cellular energy metabolism, redox biology, DNA repair, and longevity-associated signalling pathways. Researchers who buy NAD+ research grade use it to investigate sirtuin enzyme activation, PARP-mediated DNA repair, electron transport chain redox reactions, circadian rhythm regulation, mitochondrial function, and the cellular biology of ageing-associated NAD+ decline. PrimaLab Peptide supplies research-grade NAD+ at ≥99.1% purity, independently verified by HPLC with a third-party Certificate of Analysis, supplied as lyophilized powder with a 24-month sealed shelf life, in 100mg, 500mg, and 1000mg vials.

What Is NAD+?

NAD+ (Nicotinamide Adenine Dinucleotide, oxidised form) is a dinucleotide coenzyme consisting of two nucleotides joined by a phosphate bridge: one nucleotide carrying an adenine base, and one carrying a nicotinamide base. Its molecular formula is C₂₁H₂₇N₇O₁₄P₂ (MW: 663.43 g/mol, CAS: 53-84-9). The nicotinamide ring is the chemically active site — it accepts electrons (becoming NADH) and donates electrons (reverting to NAD+) in redox reactions, making NAD+/NADH the central electron carrier couple in cellular energy metabolism.

NAD+ exists in biological systems in four interconvertible forms — NAD+ (oxidised), NADH (reduced), NADP+ (phosphorylated oxidised), and NADPH (phosphorylated reduced) — each with distinct research applications. PrimaLab Peptide supplies NAD+ in its oxidised (NAD+) form — the substrate form for sirtuin deacylases and PARP enzymes, making it the appropriate form for research into these enzyme classes.

A comprehensive review of NAD+ biochemistry and its role in cellular metabolism and aging is available through NIH-published research on NAD+ in aging and metabolism.

NAD+ Research Applications

NAD+ is one of the most cited molecules in cell biology research, appearing in studies spanning energy metabolism, epigenetics, DNA repair, and ageing biology. Published research on PubMed has examined NAD+ across the following key research areas:

• Sirtuin (SIRT1-7) activation research: Sirtuins are NAD+-dependent protein deacylases that regulate histone modification, gene expression, and metabolic adaptation. As NAD+ is the essential co-substrate for all sirtuin deacylase activity, NAD+ availability directly controls sirtuin function — making it the central research tool for studying sirtuin biology, longevity-associated transcriptional regulation, and metabolic adaptation responses
• PARP enzyme and DNA repair research: PARP enzymes (Poly(ADP-ribose) polymerases) consume NAD+ to synthesise poly(ADP-ribose) chains at sites of DNA damage — a critical DNA repair signalling mechanism. NAD+ is therefore a key research substrate for studying DNA damage response, PARP activation kinetics, and the NAD+/PARP interaction relevant to genome stability research
• Electron transport chain and ATP production research: NADH (the reduced form produced during glycolysis and the TCA cycle) donates electrons to Complex I of the mitochondrial ETC, driving ATP synthesis via oxidative phosphorylation. Research into mitochondrial bioenergetics, Complex I function, and ATP production efficiency requires NAD+/NADH cycling research tools
• Circadian rhythm regulation research: The NAD+ biosynthesis pathway is directly regulated by circadian clock genes (CLOCK/BMAL1 regulate NAMPT, the rate-limiting enzyme in the NAD+ salvage pathway). Research has examined NAD+ oscillation as a key metabolic output of the circadian clock
• Mitochondrial biogenesis research: NAD+ and sirtuin activation — particularly SIRT1 and SIRT3 — are studied for their roles in mitochondrial biogenesis through PGC-1α deacetylation, relevant to exercise biology and mitochondrial health research
• Aging and cellular senescence research: NAD+ levels decline with age in most tissues — a well-documented phenomenon studied as a potential contributor to age-associated functional decline. Research programs examining NAD+ in aging biology frequently combine it with complementary longevity compounds including SS-31 (Elamipretide), Epitalon, MOTS-c, and Glutathione
• NAD+ precursor pathway research: NAD+ is used as the substrate reference standard in studies examining NAD+ biosynthetic pathways — including the salvage pathway (nicotinamide → NMN → NAD+ via NAMPT and NMNAT), the Preiss-Handler pathway (nicotinic acid → NAAD → NAD+), and the de novo pathway from tryptophan

Purity, Storage Stability & Third-Party Testing

PrimaLab Peptide’s NAD+ tests at ≥99.1% purity by HPLC, verified by a third-party COA included with every order. The testing process involves dual verification: HPLC analysis at synthesis stage and a second independent HPLC confirmation. The COA is included with every order and downloadable directly from the product page.

NAD+ stability considerations are particularly important in research:

• Oxidation sensitivity: NAD+ is susceptible to oxidative degradation — particularly by atmospheric oxygen when in solution. The lyophilized form dramatically slows oxidative degradation compared to aqueous NAD+ solutions
• Hydrolysis: NAD+ undergoes hydrolysis in neutral to alkaline aqueous solutions — degradation accelerating with increasing pH and temperature. Store lyophilized NAD+ at −20°C in a dry environment away from light and moisture
• 24-month sealed shelf life: Vacuum-sealed NAD+ vials shipped with desiccant maintain ≥99.1% purity for at least 24 months from manufacture date when stored correctly at −20°C
• Post-reconstitution stability: Once reconstituted, use within 24 hours or aliquot and refreeze — do not refreeze more than once. Stability drops significantly after reconstitution due to hydrolysis in aqueous solution

Storage & Reconstitution

• Sealed (lyophilized): −20°C in a dry environment, away from light and moisture. 24-month shelf life under these conditions. Vacuum-sealed with desiccant for transit
• After reconstitution: Use within 24 hours. If extended storage is needed, aliquot immediately and refreeze at −20°C — but do not refreeze more than once per aliquot
• Reconstitution: NAD+ is soluble in water at up to 50 mg/mL. Reconstitute with sterile water or your research buffer as required. Avoid alkaline pH conditions which accelerate hydrolysis. Use promptly after reconstitution
• Freeze-thaw cycles: Minimise — each freeze-thaw cycle introduces hydrolysis risk in the brief thawed window. Aliquot into single-use volumes before freezing to avoid repeated freeze-thaw of the same material

Product Specifications

Full Name	Nicotinamide Adenine Dinucleotide (oxidised) — NAD+
CAS Number	53-84-9
Molecular Formula	C₂₁H₂₇N₇O₁₄P₂
Molecular Weight	663.43 g/mol
Purity	≥99.1% (HPLC — third-party COA verified)
Form	Lyophilized white to off-white powder
Solubility	Water (up to 50 mg/mL)
Available Sizes	100mg, 500mg, 1000mg
Storage	−20°C, dry, away from light — 24 months sealed
Post-reconstitution stability	Use within 24 hours or aliquot and refreeze (once only)
Shipping	Vacuum-sealed with desiccant
COA	Third-party HPLC verified — included with every order
Intended Use	In vitro research only — not for human use

Frequently Asked Questions — NAD+ Research Grade

What is NAD+ used for in research?

Researchers who buy NAD+ research grade use it to investigate sirtuin (SIRT1-7) deacylase enzyme activation and downstream epigenetic regulation, PARP enzyme DNA repair signalling, electron transport chain redox reactions and ATP production, circadian rhythm metabolic regulation, mitochondrial biogenesis through PGC-1α and sirtuin pathways, cellular senescence and age-associated NAD+ decline, and NAD+ biosynthetic pathway research. Published research is available on PubMed.

Why does NAD+ degrade quickly after reconstitution?

NAD+ undergoes chemical hydrolysis in aqueous solution — the N-glycosidic bond between nicotinamide and ribose is susceptible to hydrolytic cleavage, particularly at neutral to alkaline pH and elevated temperature. This degradation produces nicotinamide and ADP-ribose, neither of which has NAD+ coenzyme activity. The lyophilized (dry) form of NAD+ is dramatically more stable than aqueous solutions because hydrolysis requires water as a reactant. Once reconstituted, use NAD+ within 24 hours or aliquot and refreeze immediately to minimise hydrolytic losses during any thawed period.

What is the difference between NAD+ and NADH in research?

NAD+ (oxidised form) and NADH (reduced form) are interconvertible redox partners in cellular metabolism. NAD+ is the substrate for sirtuin deacylases and PARP enzymes — both enzyme classes require NAD+ specifically, not NADH. NADH is the electron-carrying reduced form that feeds electrons to the ETC at Complex I. Research into sirtuin biology, PARP-mediated DNA repair, and NAD+ availability studies should use the NAD+ (oxidised) form supplied by PrimaLab Peptide. Research examining NADH-specific ETC function requires the NADH form.

Is NAD+ from PrimaLab Peptide independently verified?

Yes. Every batch of NAD+ is tested by HPLC at synthesis and independently confirmed by a second HPLC analysis at a third-party laboratory, producing a COA with the confirmed purity figure (≥99.1%) that is included with every order. Vials are vacuum-sealed and shipped with desiccant to maintain purity throughout transit. The 24-month sealed shelf life at −20°C is validated under these storage conditions.

What compounds are commonly studied alongside NAD+ in longevity research?

Researchers buying NAD+ research grade for longevity and cellular aging research frequently explore complementary compounds from PrimaLab Peptide’s Wellness & Longevity category, including SS-31 (Elamipretide) (mitochondrial cardiolipin stabilisation), Epitalon (telomerase activation and longevity pathways), MOTS-c (mitochondria-derived AMPK activation peptide), and Glutathione (master antioxidant and redox balance research). Browse the complete Wellness & Longevity category.

What vial sizes are available, and is purity consistent across sizes?

NAD+ is available from PrimaLab Peptide in 100mg, 500mg, and 1000mg lyophilized vials. All sizes are manufactured to the same ≥99.1% purity standard and verified by the same HPLC testing process — there is no difference in quality between vial sizes. The COA and purity standard are consistent regardless of the vial size ordered.

Related Research Compounds

Researchers buying NAD+ research grade for longevity, sirtuin, and cellular energy research frequently explore:

• SS-31 (Elamipretide) — mitochondria-targeted cardiolipin-binding tetrapeptide for ETC and ROS research
• Epitalon — AEDG tetrapeptide for telomerase activation and longevity pathway research
• MOTS-c — mitochondria-derived peptide for AMPK activation and metabolic regulation
• Glutathione (GSH) — master antioxidant for redox balance and cellular protection research
• 5-Amino-1MQ — NNMT inhibitor studied for effects on NAD+ precursor availability and metabolic research

Browse our complete Wellness & Longevity category. Bulk researchers can apply for discounted rates through our Wholesale Portal.

⚠️ For in-vitro research only. Not intended for human use. Not a drug, supplement, or medical treatment. These statements have not been evaluated by any regulatory authority. Researchers are solely responsible for compliance with all applicable local, national, and international regulations.